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From the movie "A Night at the Roxbury".
Originating from a skit on "Saturday Night Live". The Roxbury Guys are brothers who want to go clubbing at the popular Night spot The Roxbury, but are constantly denied entry.
The car scene has the song "What is Love" by Haddaway playing, and the guys enjoy themselves by moving their head to the beat.
Tuned into a moving gif, it is parodied a lot by pasting cutout heads over the actors.